Ziwei(Zoe) Wu | 吴梓微

Ziwei(Zoe) Wu | 吴梓微

We all become cosmic dust, eventually. May my existence find meaning, sincerely.

Undergraduate Comprehensive Experiments in Ecology in 2022 autumn semester

Undergraduate course, Sun Yat-sen University, School of Ecology, 2023

Responsible for the following components:

  1. Population Genetic Analysis of Pomacea spp. in Subtropical Regions.
    •   Extraction of DNA from 16 individuals of the apple snail species Pomacea canaliculata, followed by the construction of second-generation genomic libraries (using the TN5 transposase method) for population genetics analysis of the apple snail population.
  2. Research on the Adaptability of Apple Snails: Transcriptomic Analysis in Low-Temperature Environments.
    •   Collected samples of apple snails, dividing them into a control group (6 individuals) and a low-temperature experimental group (3 individuals). The control group was kept at a constant 25 degrees Celsius for 6 days. The low-temperature group experienced a 3-day exposure at 25 degrees Celsius followed by a gradual decrease to 5 degrees Celsius for another 3 days. We extracted RNA from the muscle tissue of individuals in both groups, initially using the Trizol method, and then converted it to cDNA using SMART-seq2. Subsequently, a second-generation genome library was constructed (utilizing the Tn5 technique) to explore further the adaptive mechanisms of apple snails under low-temperature stress conditions.
  3. Metagenomic Study of the Subterranean Termite (Odontotermes formosanus) and its Ecological Function in Lignocellulose Degradation.
    •   Sequencing of the microbial community within termite nest environments (second and third-generation sequencing). Utilizing second-generation (as described above) and third-generation (ONT) sequencing technologies to construct environmental microbial community libraries and sequence them, revealing microbial diversity and potential functions within termite nests.
  4. Bioinformatics component (ATAC-seq, RNA-seq, Metagenomic Analysis):
    •   Primarily responsible for instructing RNA-seq data processing and analysis while also providing assistance in guiding data analysis for ATAC-seq and Metagenomic studies.

Undergraduate Biochemical Experiments in 2022 spring semester

Undergraduate course, Sun Yat-sen University, School of Ecology, 2022

Responsible for reagent preparation and conducting preliminary experiments, this module comprises a total of eight distinct laboratory exercises:

  1. Experiment One: Thin-Layer Chromatography of Amino Acids
  2. Experiment Two: Determination of Catalase Activity
  3. Experiment Three: Colorimetric Quantification of Total and Soluble Sugars in Plant Tissues using the Anthrone Method
  4. Experiment Four: Urease Km Value Determination via a Simplified Approach
  5. Experiment Five: Measurement of Vitamin C Content - 2,6-Dichlorophenol Indophenol Method
  6. Comprehensive Experiment (Part One): Extraction and Partial Purification of Sucrase
  7. Comprehensive Experiment (Part Two): Assessment of Sucrase Activity and Protein Content
  8. Comprehensive Experiment (Part Three): SDS-PAGE Electrophoresis Analysis for Product Separation

Undergraduate Comprehensive Experiment in Ecology in 2021 autumn semester

Undergraduate course, Sun Yat-sen University , School of Ecology, 2022

I am primarily responsible for preparing experimental reagents, conducting preliminary experiments, and guiding students. The course encompasses the following components:

  1. Construction of second-generation genomic libraries using DNA from crucian carp (utilizing TN5 transposase method).
  2. Crucian carp gill ATAC-seq Library Construction:   Employing ATAC-seq technology to construct second-generation DNA libraries, investigating open chromatin regions, and unraveling gene expression regulation mechanisms.
  3. Construction of gill TRACE-Seq RNA-seq libraries:   Utilizing TRACE-Seq to convert RNA from fish gills into cDNA and constructing cDNA libraries using the TN5 transposase method.
  4. Environmental Microbial Second and Third-generation Library Sequencing:   Applying second-generation (Tn5) and third-generation (ONT) sequencing technologies to construct environmental microbial community libraries and sequence them, revealing microbial diversity and potential functions.
  5. Bioinformatics component(ATAC-seq, RNA-seq, Metagenomic Analysis):   Responsible for instructing RNA-seq data processing and analysis.