Undergraduate Biochemical Experiments in 2022 spring semester

Undergraduate course, Sun Yat-sen University, School of Ecology, 2022

Responsible for reagent preparation and conducting preliminary experiments, this module comprises a total of eight distinct laboratory exercises:

  1. Experiment One: Thin-Layer Chromatography of Amino Acids
  2. Experiment Two: Determination of Catalase Activity
  3. Experiment Three: Colorimetric Quantification of Total and Soluble Sugars in Plant Tissues using the Anthrone Method
  4. Experiment Four: Urease Km Value Determination via a Simplified Approach
  5. Experiment Five: Measurement of Vitamin C Content - 2,6-Dichlorophenol Indophenol Method
  6. Comprehensive Experiment (Part One): Extraction and Partial Purification of Sucrase
  7. Comprehensive Experiment (Part Two): Assessment of Sucrase Activity and Protein Content
  8. Comprehensive Experiment (Part Three): SDS-PAGE Electrophoresis Analysis for Product Separation